1锟斤拷锟斤拷B

锟叫囷拷锟絵锟斤拷锟窖筹拷锟斤拷CHO锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟酵硷拷锟斤拷锟絯锟絜锟絚锟截讹拷锟斤拷锟截組锟斤拷锟斤拷锟斤拷锟絘锟斤拷锟斤拷锟絇锟斤拷锟斤拷锟斤拷铣锟斤拷锟斤拷锟斤拷蜖I锟紹(y锟斤拷ng)锟斤拷锟絈取锟絈锟节硷拷锟斤拷锟斤拷小锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷小锟斤拷锟杰碉拷锟斤拷锟紹(y锟斤拷ng)锟斤拷B透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷影锟�锟斤拷锟斤拷知锟竭滲锟絣锟斤拷锟斤拷锟斤拷锟铰硷拷锟斤拷锟絯锟絜锟斤拷锟斤拷锟斤拷

锟斤拷锟斤拷锟斤拷小锟斤拷锟截讹拷锟斤拷锟斤拷锟絴锟斤拷锟絘锟斤拷锟侥決锟斤拷锟斤拷锟斤拷锟斤拷锟組锟斤拷每锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紸锟轿的硷拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絘锟斤拷锟斤拷锟斤拷G2/M锟节的硷拷锟斤拷锟斤拷锟紽(xi锟斤拷n)锟斤拷锟斤拷叩锟斤拷锟斤拷a锟斤拷锟斤拷
锟斤拷某些锟叫撅拷锟斤拷锟斤拷锟轿度碉拷锟斤拷锟接帮拷锟絊锟斤拷锟斤拷锟斤拷锟絴锟絴锟斤拷锟斤拷變锟斤拷锟斤拷锟截別锟斤拷锟斤拷锟角伙拷锟斤拷锟斤拷锟斤拷锟斤拷锟窖斤拷(j锟斤拷ng)锟絣(f锟斤拷)锟紽(xi锟斤拷n)锟竭滲透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟皆斤拷锟斤拷Fc锟节合碉拷锟阶碉拷N-锟斤拷锟斤拷锟斤拷锟皆拷锟斤拷锟絯锟斤拷锟斤拷液锟结含锟斤拷锟侥憋拷锟斤拷锟斤拷锟紷锟斤拷锟斤拷通锟絕锟斤拷锟斤拷锟斤拷藟A锟斤拷锟斤拷锟斤拷锟斤拷
同锟斤拷锟斤拷锟斤拷锟絘锟较凤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟紸锟斤拷锟斤拷锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟�60-120mOsm/kg锟斤拷锟斤拷锟斤拷未锟斤拷旨庸锟斤拷木锟斤拷堑姆锟斤拷凇锟�

2锟斤拷锟結锟斤拷锟斤拷討論

一锟斤拷锟竭滲透锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絃锟酵达拷锟絰锟斤拷影锟�

锟紻1锟斤拷锟叫囷拷锟絵锟斤拷锟窖筹拷锟斤拷CHO锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷L锟斤拷锟斤拷锟絚锟斤拷锟斤拷锟斤拷B透锟斤拷锟侥猴拷锟斤拷(sh锟斤拷)锟絇系锟斤拷

锟揭傦拷锟节接種前锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟叫硷拷锟斤拷NaCl锟斤拷锟絘锟斤拷C锟斤拷锟皆獲锟斤拷320锟斤拷500 mOsm kg?1之锟絞锟侥筹拷始锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷值锟斤拷锟斤拷D1锟斤拷示锟斤拷锟斤拷锟紷锟缴種锟斤拷r锟斤拷锟斤拷锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟斤拷锟接岋拷锟斤拷锟斤拷锟斤拷囟锟斤拷锟斤拷锟斤拷锟斤拷L锟斤拷锟紷锟斤拷锟斤拷锟酵★拷锟缴種處锟斤拷锟斤拷式锟斤拷锟斤拷锟斤拷锟斤拷L锟绞差異锟給锟統(t锟斤拷ng)計锟絎锟斤拷锟絰锟斤拷p>0.05锟斤拷锟斤拷

锟斤拷同锟紹透锟斤拷锟絣锟斤拷锟铰硷拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷(sh锟斤拷)锟絊锟絩锟絞锟斤拷變锟斤拷锟斤拷锟斤拷

锟筋細锟斤拷锟杰讹拷锟斤拷锟斤拷锟斤拷D2A锟斤拷示锟絊锟斤拷锟絵锟斤拷锟絘锟较碉拷锟斤拷锟接滲透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷樱锟斤拷锟斤拷L锟斤拷锟斤拷锟斤拷锟紷锟斤拷锟斤拷锟斤拷锟紷些锟絣(f锟斤拷)锟紽(xi锟斤拷n)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絘锟较曪拷锟斤拷锟斤拷锟斤拷锟狡硷拷锟斤拷锟斤拷锟絃锟斤拷锟节ｏ拷锟斤拷锟斤拷锟絘锟斤拷C锟斤拷锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟絖锟斤拷500 mOsm kg?1锟斤拷锟斤拷r锟铰匡拷锟斤拷锟斤拷锟斤拷乜锟斤拷锟斤拷@一锟絚锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟絕锟斤拷锟斤拷锟斤拷锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷值锟斤拷锟接ｏ拷锟紻2B锟斤拷锟斤拷锟斤拷要锟斤拷锟斤拷锟节硷拷锟斤拷锟斤拷锟斤拷锟结、Na+锟斤拷K+锟侥積锟桔ｏ拷锟紻2锟斤拷锟街別锟斤拷E锟斤拷G锟斤拷H锟斤拷锟斤拷

直锟斤拷锟斤拷5锟斤拷锟斤拷平锟斤拷锟斤拷锟斤拷直锟斤拷锟絚锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絇锟斤拷锟紻2C锟斤拷锟斤拷锟斤拷值锟节接種锟斤拷24小锟絩锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟截別锟角岋拷锟斤拷锟斤拷锟斤拷锟絘锟斤拷C锟斤拷锟絕410 mOsm kg?1锟絚锟斤拷锟秸組锟斤拷确浅锟斤拷锟斤拷@锟斤拷然锟斤拷锟斤拷锟侥碉拷6锟斤拷锟絖始锟斤拷锟斤拷锟斤拷锟秸猴拷锟斤拷锟斤拷NaCl锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟叫碉拷锟斤拷锟斤拷锟角和谷帮拷锟斤拷锟斤拷P锟絀锟絀锟紹(y锟斤拷ng)锟斤拷锟絴锟絖始锟侥盡锟絩锟斤拷趨锟捷發(f锟斤拷)锟斤拷锟斤拷變锟斤拷锟斤拷锟紻2F锟斤拷2I锟斤拷锟斤拷

锟斤拷锟斤拷锟�(锟紻2D)锟斤拷锟斤拷u锟斤拷锟接碉拷趨锟斤拷锟斤拷锟斤拷只锟斤拷3锟絅锟絣锟斤拷锟斤拷锟絕5mM锟斤拷锟斤拷該锟斤拷锟斤拷系[36]锟斤拷锟斤拷锟紷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷然锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷r锟斤拷锟斤拷锟紷一锟街碉拷锟斤拷锟斤拷o止锟节伙拷锟斤拷锟斤拷锟斤拷B(y锟斤拷ng)锟斤拷锟杰匡拷锟杰诧拷锟絧锟斤拷锟絖锟斤拷锟竭峰細锟斤拷锟杰度★拷锟紻2E锟斤拷F锟街別锟紷示锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷堑姆植锟斤拷锟斤拷锟斤拷锟角帮拷邚漠a锟斤拷锟斤拷锟斤拷锟侥碉拷锟紻變锟紿锟节岋拷锟秸猴拷320mOsmkg-1锟絣锟斤拷锟斤拷锟組锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷B(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟絕锟斤拷锟叫憋拷锟紽(xi锟斤拷n)锟斤拷锟斤拷锟絤(x锟斤拷)锟斤拷锟斤拷锟斤拷a锟斤拷锟斤拷锟斤拷一些锟斤拷锟斤拷NaCl锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟狡猴拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟皆�锟斤拷锟斤拷锟斤拷锟斤拷a锟斤拷锟斤拷锟斤拷锟侥碉拷锟紻變锟斤拷锟絁锟斤拷取锟絈锟斤拷锟斤拷锟紹(y锟斤拷ng)液锟斤拷pH值锟斤拷

锟紻3 锟斤拷同锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟絣锟斤拷锟铰ｏ拷A锟斤拷锟斤拷锟斤拷锟斤拷B锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷c锟斤拷锟饺帮拷锟结、锟斤拷D锟斤拷锟饺帮拷锟斤拷锟斤拷锟酵ｏ拷E锟斤拷锟斤拷锟斤拷锟斤拷囟锟斤拷z取/锟絘锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷未锟絘锟斤拷锟缴檢锟統水平锟侥谷帮拷锟斤拷锟斤拷锟斤拷锟絗锟斤拷l锟斤拷示锟缴傦拷平锟叫岋拷锟侥標滐拷偏锟筋。锟斤拷锟斤拷锟紷锟斤拷锟皆凤拷锟斤拷锟斤拷*p<0.05锟斤拷锟斤拷锟斤拷锟絆锟斤拷同锟斤拷锟街拷g每锟斤拷變锟斤拷锟侥凤拷锟斤拷锟斤拷锟�.

锟紻3锟紷示锟斤拷指锟斤拷(sh锟斤拷)锟斤拷锟絃锟紸锟轿ｏ拷锟斤拷锟斤拷锟斤拷(j锟斤拷)锟斤拷0锟斤拷锟斤拷锟斤拷6锟斤拷臄锟�(sh锟斤拷)锟斤拷(j锟斤拷)計锟姐）锟揭傦拷锟絕锟届到锟剿恒定锟侥滲透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷值锟斤拷锟絚锟斤拷锟秸組锟斤拷锟�锟斤拷锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷囟锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷识锟斤拷^锟斤拷锟斤拷锟組锟杰憋拷锟紽(xi锟斤拷n)锟斤拷锟絕锟酵碉拷锟截讹拷锟斤拷锟斤拷锟斤拷锟絃锟绞★拷锟給論锟絊锟斤拷母邼B處锟斤拷锟斤拷锟�锟斤拷锟絊锟斤拷锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟较碉拷锟斤拷锟斤拷a锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紷锟斤拷锟斤拷锟給論锟絋锟斤拷锟斤拷锟斤拷锟斤拷锟�锟斤拷碳锟斤拷锟絰锟斤拷锟斤拷锟斤拷帧锟斤拷锟角帮拷呀锟�(j锟斤拷ng)锟斤拷锟�锟斤拷锟斤拷锟絕锟竭的氾拷锟斤拷锟斤拷锟斤拷锟角濓拷锟斤拷锟�锟斤拷锟斤拷锟斤拷锟斤拷锟较碉拷锟斤拷锟斤拷a锟斤拷锟斤拷锟斤拷锟斤拷锟铰岋拷锟较ｏ拷锟揭傦拷锟侥岋拷锟結锟斤拷指锟斤拷锟斤拷同锟侥憋拷锟斤拷锟斤拷锟斤拷锟絕使锟斤拷锟斤拷同锟絋锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷之锟絞锟侥氾拷锟斤拷锟斤拷锟斤拷锟角濓拷龋锟斤拷D2F锟斤拷锟斤拷锟揭傦拷锟絣(f锟斤拷)锟紽(xi锟斤拷n)锟斤拷锟斤拷锟角濓拷锟皆斤拷撸锟斤拷诩锟斤拷锟斤拷锟斤拷L锟节ｏ拷锟斤拷锟斤拷某锟绞硷拷m锟斤拷锟斤拷锟斤拷锟絊锟斤拷锟斤拷指锟斤拷(sh锟斤拷)锟斤拷锟斤拷锟斤拷锟絃锟斤拷锟斤拷锟斤拷锟斤拷漠a锟斤拷锟绞撅拷越锟竭ｏ拷锟紻3E锟斤拷锟斤拷锟紷锟絅锟叫烇拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷锟絕锟絤(x锟斤拷)锟斤拷锟斤拷锟斤拷锟斤拷锟紹透锟斤拷锟絣锟斤拷锟铰ｏ拷锟斤拷锟秸猴拷370mOsm kg?1 NaCl锟斤拷锟侥兩種锟斤拷锟紹(y锟斤拷ng)锟斤拷锟紷示锟斤拷锟斤拷锟斤拷锟饺的匡拷锟斤拷锟铰斤拷锟斤拷锟紷锟斤拷锟斤拷锟节硷拷锟斤拷锟斤拷锟斤拷锟絰锟絘锟斤拷锟斤拷锟斤拷碳源锟斤拷锟铰內伙拷锟斤拷锟铰ｏ拷锟紻2E锟斤拷锟斤拷锟节硷拷锟斤拷NaCl锟斤拷锟斤拷邼B透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟絩锟斤拷锟斤拷锟絘锟斤拷锟斤拷锟斤拷锟斤拷锟絵锟斤拷一锟斤拷锟斤拷锟狡猴拷锟斤拷锟斤拷锟角酵斤拷锟斤拷锟斤拷膩锟皆�锟斤拷锟斤拷前锟叫堬拷锟斤拷Q锟斤拷锟饺帮拷锟斤拷锟矫革拷锟斤拷锟斤拷诎锟斤拷Y锟斤拷锟斤拷锟斤拷锟结供碳锟酵碉拷锟斤拷锟斤拷知锟紷些锟斤拷锟斤拷锟絚CHO锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟狡的达拷锟絰锟斤拷锟斤拷锟斤拷锟斤拷趣锟斤拷锟斤拷锟斤拷锟斤拷锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷值锟斤拷410-420mOsm kg?1锟斤拷锟斤拷锟饺時锟斤拷锟节兩種锟竭滲锟絋锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟侥產锟斤拷锟绞沒锟斤拷锟紷锟斤拷锟筋異锟斤拷然锟斤拷锟斤拷锟紷锟絅變锟斤拷锟斤拷460锟紺470 mOsm kg?1锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷NaCl锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷F(xi锟斤拷n)锟斤拷锟紷锟斤拷锟斤拷锟竭的帮拷锟斤拷锟斤拷锟绞ｏ拷p<0.05锟斤拷锟斤拷锟斤拷锟絯锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷NaCl锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷a锟斤拷C锟侥岋拷锟斤拷锟节革拷锟斤拷陌锟斤拷锟斤拷D3A锟斤拷锟斤拷锟紷锟絚锟斤拷锟皆的谷帮拷锟斤拷锟斤拷锟斤拷锟絘锟斤拷锟斤拷一锟铰ｏ拷锟斤拷锟斤拷使锟矫帮拷锟较成和凤拷锟节谷帮拷锟斤拷锟斤拷锟斤拷锟斤拷锟绞革拷锟斤拷前锟竭ｏ拷锟紻3D锟斤拷锟斤拷然锟斤拷锟斤拷锟饺帮拷锟斤拷臄z取/锟斤拷锟絘锟斤拷锟绞诧拷锟斤拷锟斤拷循锟斤拷同锟斤拷模式锟斤拷460锟紺470 mOsm kg?1锟斤拷锟斤拷锟饺碉拷NaCl锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟侥谷帮拷锟斤拷锟斤拷锟斤拷锟絘锟斤拷C锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷诠劝锟斤拷锟�锟斤拷锟絙锟斤拷锟絘锟斤拷C锟斤拷锟斤拷锟斤拷锟斤拷锟结，锟揭傦拷锟斤拷锟絆锟紷些锟斤拷锟紹(y锟斤拷ng)锟斤將锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紻锟斤拷锟斤拷劝锟斤拷锟�锟斤拷然锟斤拷锟斤拷锟节合成谷帮拷锟斤拷锟斤拷锟斤拷锟斤拷趣锟斤拷锟斤拷锟斤拷500 mOsm kg?1锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷F(xi锟斤拷n)锟斤拷同锟斤拷锟斤拷锟紽(xi锟斤拷n)锟斤拷锟斤拷锟酵谷帮拷锟斤拷a锟斤拷锟斤拷锟接ｏ拷锟饺帮拷锟斤拷锟斤拷锟斤拷锟节減锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紷些锟竭滲透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟絀锟紹(y锟斤拷ng)锟絊锟斤拷锟侥條锟斤拷锟斤拷锟叫诧拷同锟侥达拷锟絰途锟斤拷锟斤拷

锟斤拷锟斤拷锟竭滲锟斤拷锟斤拷锟轿度猴拷锟截拷锟皆匡拷锟絯锟斤拷锟絘锟绞硷拷锟角伙拷锟斤拷锟斤拷影锟�

锟紻4锟紷示锟斤拷锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟秸獲锟絩锟斤拷mAb锟轿度和革拷锟皆碉拷锟斤拷锟絯锟筋細锟斤拷锟杰度ｏ拷IVCD锟斤拷值锟斤拷锟斤拷叩味葹锟�410 mOsm kg?1锟斤拷锟斤拷锟斤拷锟絘锟斤拷C也锟紷示锟斤拷锟斤拷叩锟絀VCD锟斤拷锟組锟杰猴拷锟斤拷锟絚锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷]锟斤拷锟紷锟斤拷锟筋異锟斤拷锟斤拷锟斤拷一锟剿ｏ拷500 mOsm kg?1锟絘锟斤拷C锟斤拷锟紹(y锟斤拷ng)锟斤拷锟絀VCD锟斤拷mAb锟轿讹拷锟斤拷锟�锟�锟斤拷锟斤拷膩锟斤拷f锟斤拷锟轿度达拷锟斤拷锟斤拷循锟絚IVCD锟斤拷同锟斤拷趨锟斤拷锟斤拷470 mOsm kg?1 NaCl锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟�锟斤拷锟侥圖5锟叫碉拷锟截讹拷mAb锟斤拷锟絘锟斤拷锟揭傦拷锟斤拷锟皆匡拷锟斤拷锟斤拷锟斤拷锟斤拷锟叫條锟斤拷锟斤拷锟斤拷470 mOsm kg?1 NaCl锟斤拷锟紹(y锟斤拷ng)锟斤拷锟絤Ab锟斤拷锟斤拷锟斤拷锟斤拷摺锟斤拷@锟斤拷锟紿锟斤拷锟节岋拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絘锟斤拷C锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷趣锟斤拷锟角ｏ拷锟絘锟斤拷NaCl锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟紷示锟斤拷锟截讹拷mAb锟斤拷锟絘锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟紷锟絚锟紹透锟斤拷值锟斤拷锟斤拷锟斤拷锟絇锟斤拷锟斤拷锟斤拷锟斤拷锟絘锟斤拷C锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟絅锟斤拷同锟侥滲透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷值之锟絞锟絔锟叫統(t锟斤拷ng)計锟絎锟较碉拷锟紷锟斤拷锟筋異锟斤拷锟紷锟斤拷锟斤拷源锟节猴拷一锟絅锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絊锟饺高ｏ拷锟斤拷锟絀锟紹(y锟斤拷ng)锟斤拷锟絴锟斤拷锟斤拷锟斤拷效锟斤拷锟絕锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷470 mOsm kg?1 NaCl锟斤拷410 mOsm kg?1锟絘锟斤拷C锟斤拷锟紹(y锟斤拷ng)锟斤拷锟节碉拷6锟斤拷锟斤拷锟紽(xi锟斤拷n)锟斤拷锟斤拷锟紷锟斤拷锟斤拷募锟斤拷锟街憋拷锟�锟斤拷锟斤拷D2C锟斤拷示锟斤拷

锟缴種锟竭滲锟絋锟斤拷锟斤拷锟斤拷锟斤拷锟秸獲锟絩mAb锟角伙拷锟斤拷锟斤拷影锟斤拷锟紻6锟斤拷示锟斤拷锟斤拷锟斤拷锟斤拷NaCl锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟叫ｏ拷锟斤拷锟角分诧拷锟絔锟叫統(t锟斤拷ng)計锟絎锟较碉拷锟紷锟斤拷锟筋異锟斤拷p>0.05锟斤拷锟斤拷然锟斤拷锟斤拷锟斤拷锟接革拷锟斤拷锟絘锟斤拷C锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟紷示锟斤拷锟絊锟斤拷锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷G0F锟铰斤拷锟斤拷410锟斤拷500 mOsm kg?1之锟絞锟侥差異锟斤拷18%锟斤拷锟斤拷锟紷锟斤拷锟絊锟斤拷G0锟斤拷锟斤拷锟接ｏ拷410锟斤拷500 mOsm kg?1之锟絞锟侥差異锟斤拷10%锟斤拷锟酵帮拷锟斤拷锟角伙拷锟斤拷锟劫分比碉拷锟絕小锟斤拷锟接ｏ拷G1F锟斤拷G10F锟斤拷G2F锟斤拷锟斤拷然锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟�锟斤拷锟斤拷锟紽(xi锟斤拷n)锟斤拷锟斤拷锟斤拷锟絅锟斤拷锟絘锟斤拷锟斤拷410 mOsm kg?1锟絘锟斤拷c锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷也锟紷示锟斤拷锟角帮拷锟斤拷锟角伙拷锟斤拷锟侥百分憋拷锟斤拷锟斤拷锟斤拷锟斤拷一锟斤拷锟斤拷锟斤拷锟斤拷锟絯锟较成和凤拷锟节的革拷锟斤拷锟绞匡拷锟斤拷锟斤拷锟斤拷锟剿匡拷锟絯锟节高狅拷锟斤拷锟絯锟叫碉拷停锟斤拷锟絩锟絞锟斤拷锟斤拷私锟斤拷锟斤拷锟截撠�mAb锟斤拷锟斤拷锟角伙拷锟斤拷锟斤拷b-1,4-锟斤拷锟斤拷锟角伙拷锟紻锟斤拷酶锟斤拷b4GalT锟斤拷锟斤拷锟斤拷锟角碉拷锟組一锟斤拷锟接癸拷锟教度★拷锟絚锟紸锟节的非帮拷锟斤拷锟角伙拷锟斤拷锟結锟斤拷锟侥分诧拷锟斤拷锟斤拷锟洁反锟斤拷锟斤拷锟侥巖锟斤拷锟角伙拷锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟叫憋拷锟紽(xi锟斤拷n)锟斤拷锟斤拷锟诫不锟斤拷锟斤拷锟叫為，锟斤拷锟斤拷锟斤拷锟斤拷锟絘锟斤拷C锟斤拷锟絳锟斤拷(ji锟斤拷)锟紹透锟斤拷锟斤拷锟斤拷D6锟斤拷示锟斤拷锟斤拷邼B透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟絣锟斤拷锟斤拷500 mOsm kg?1锟絘锟斤拷C锟斤拷锟侥猴拷锟侥巖锟斤拷锟角伙拷锟斤拷水平锟斤拷锟紷锟斤拷锟斤拷410 mOsm kg?1锟絘锟斤拷C锟斤拷品锟斤拷p<0.05锟斤拷锟斤拷锟組锟斤拷锟斤拷锟截拷锟皆匡拷锟絯锟斤拷锟絘锟斤拷锟絕锟斤拷锟斤拷锟斤拷锟斤拷锟絕锟斤拷Y锟斤拷锟斤拷锟杰憋拷锟斤拷锟斤拷锟斤拷锟斤拷锟絕锟竭的滲透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷水平锟斤拷a-1,6-锟絩锟斤拷锟角伙拷锟紻锟斤拷酶锟斤拷FucT锟斤拷酶锟斤拷锟斤拷锟杰碉拷锟斤拷锟狡伙拷锟斤拷锟�Fut8锟斤拷锟斤拷锟絖锟絧锟劫ｏ拷锟斤拷锟絆锟斤拷锟侥巖锟斤拷锟角伙拷锟侥癸拷锟斤拷锟斤拷GDP锟絩锟斤拷锟角碉拷水平锟斤拷锟杰滲透锟斤拷變锟斤拷锟斤拷影锟�锟斤拷

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锟斤拷锟叫撅拷锟斤拷CHO锟斤拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟絖始锟絩锟斤拷锟斤拷锟絘锟较猴拷锟絵锟絋锟斤拷锟侥高滲锟皆碉拷影響猴拷锟斤拷锟揭傦拷锟斤拷锟斤拷锟斤反锟斤拷锟斤拷锟斤拷锟絕锟斤拷锟斤拷一锟組锟斤拷锟酵碉拷锟絘锟斤拷CHO锟斤拷锟斤拷锟斤拷锟斤拷锟竭革拷锟矫地达拷锟斤拷锟剿碉拷锟酵碉拷锟斤拷锟絘锟絣锟斤拷锟斤拷锟斤拷锟紷锟絅锟絣锟斤拷锟斤拷锟斤拷锟斤拷锟节營锟紹(y锟斤拷ng)锟斤拷锟絴锟斤拷锟絘锟斤拷痛锟斤拷x锟斤拷漠a锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷B透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟饺筹拷始值锟斤拷锟斤拷锟斤拷70%锟斤拷锟斤拷前锟叫堬拷锟斤拷Q锟斤拷锟紷锟斤拷锟斤拷锟铰硷拷锟斤拷锟絯锟絜锟斤拷锟斤拷锟侥憋拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤反锟斤拷锟斤拷锟絟(hu锟斤拷n)锟斤拷锟斤拷锟斤拷锟揭傦拷锟斤拷锟斤拷锟斤拷效锟截匡拷锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟�pH锟斤拷锟斤拷也锟斤拷锟斤拷探锟斤拷锟斤拷指锟斤拷(sh锟斤拷)锟斤拷锟絃锟斤拷锟絞锟斤拷锟截讹拷锟紻锟狡碉拷锟截和低溫條锟斤拷锟斤拷影锟�锟斤拷锟紷锟斤拷锟斤拷锟絃锟斤拷锟紹(y锟斤拷ng)锟絩锟絞锟斤拷锟斤拷弋a品锟轿度的筹拷锟矫诧拷锟斤拷锟斤拷锟斤拷锟揭傦拷锟斤拷锟斤拷锟斤反锟斤拷锟斤拷锟叫撅拷锟叫ｏ拷锟斤拷锟絰锟斤拷36.5锟斤拷r锟斤拷锟斤拷锟絊锟斤拷 锟斤拷锟斤拷锟斤拷锟斤拷锟角猴拷锟斤拷锟斤拷锟斤拷弑锟斤拷趤锟斤拷蜏锟斤拷赂锟斤拷锟斤拷妆锟斤拷锟斤拷锟�锟斤拷锟紷锟斤拷锟铰滲透锟斤拷摩锟斤拷锟斤拷锟斤拷锟�36.5锟絩锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷同锟斤拷锟絘锟斤拷計锟斤拷锟斤拷锟紻7a锟斤拷锟斤拷锟斤拷平锟斤拷锟斤拷锟斤拷锟斤拷小锟絃锟狡烇拷B透锟斤拷锟斤拷鹊暮锟斤拷锟�(sh锟斤拷)锟斤拷锟絝锟斤拷锟剿滲透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷葘锟斤拷锟斤拷锟斤拷w锟絜锟斤拷影響ｏ拷锟紻7b锟斤拷锟斤拷

锟結锟斤拷

锟斤拷CHO锟斤拷锟斤拷锟斤拷Fed-Batch锟斤拷锟紹(y锟斤拷ng)模式锟铰ｏ拷锟絘锟较碉拷锟斤拷锟接和达拷锟絰锟絘锟斤拷姆e锟桔岋拷锟铰猴拷锟斤拷锟斤拷锟紹(y锟斤拷ng)液锟斤拷锟叫硷拷锟斤拷锟斤拷B透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷
通锟絕NaCl锟絳锟斤拷(ji锟斤拷)锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷470 mOsm/kg锟絩锟斤拷锟斤拷锟絯锟絘锟绞和滴度撅拷锟叫达拷锟組锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷通锟絕锟絘锟斤拷Feed C锟斤拷锟斤拷锟�mAb锟絘锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絃锟斤拷锟斤拷锟紷锟斤拷锟斤拷锟斤拷锟揭岋拷锟铰帮拷锟斤拷锟角伙拷锟斤拷水平锟斤拷锟斤拷锟紷锟斤拷锟斤拷锟斤拷锟斤拷

3锟斤拷锟結論

锟节憋拷锟叫撅拷锟斤拷锟斤拷锟揭傦拷锟絲锟統锟剿高滲锟絣锟斤拷锟斤拷CHO锟斤拷锟斤拷锟斤拷锟絃锟斤拷锟斤拷锟斤拷锟絯锟絜锟酵匡拷锟絯锟絘锟斤拷锟斤拷影锟�锟斤拷锟揭傦拷锟斤拷锟絕锟剿兩種锟絋锟斤拷锟竭滲锟皆的凤拷锟斤拷锟斤拷锟斤拷锟斤拷NaCl锟斤拷锟斤拷锟斤拷锟絘锟斤拷C锟斤拷锟紹透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷锟絃锟酵匡拷锟絯锟轿度碉拷影響程度差異锟杰达拷锟斤拷取锟絈锟节滲透锟斤拷锟斤拷值锟皆硷拷锟斤拷锟接滲透锟斤拷锟斤拷摩锟斤拷锟斤拷葷B透锟斤拷锟斤拷摩锟斤拷锟紹透锟斤拷锟斤拷锟侥凤拷锟斤拷锟斤拷锟紷锟斤拷锟斤拷锟�锟斤拷锟組锟杰高滲锟絣锟斤拷锟斤拷锟斤拷锟叫條锟斤拷锟铰讹拷锟斤拷锟斤拷锟斤拷囟锟斤拷锟斤拷w锟斤拷锟斤拷锟絘锟斤拷锟斤拷锟斤拷锟斤拷锟斤拷也锟斤拷锟斤拷锟絃锟絘锟斤拷锟剿诧拷锟斤拷影锟�锟斤拷锟紷锟斤拷味锟斤拷锟斤拷某些锟斤拷r锟铰匡拷锟轿度碉拷锟节岋拷锟斤拷锟斤拷锟斤拷锟斤拷锟紷訝锟斤拷锟斤拷锟斤拷锟絚锟絘锟斤拷锟絘锟斤拷C锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷锟�锟斤拷锟絘锟斤拷NaCl锟斤拷锟斤拷锟紹(y锟斤拷ng)锟斤拷锟斤拷F(xi锟斤拷n)锟斤拷锟斤拷一锟铰碉拷锟叫為，锟紷锟斤拷锟斤拷锟絕锟斤拷锟絀锟紹(y锟斤拷ng)锟斤拷锟斤拷锟接岋拷锟斤拷锟絃锟斤拷锟绞的诧拷锟斤拷影锟�锟斤拷